All the Alexa Fluor ® dyes are sulfonated forms of different basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamin (e.g. A significant advantage of long wavelength dyes such as Alexa Fluor 647 is the low autofluorescence of biological specimens in this region of the spectrum. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. © 2020 BD. 3 Chemical and Physical Properties Expand this section. You appear to be visiting this site from the UK. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647 provides for maximum color separation. 2 Names and Identifiers Expand this section. Alexa Fluor 594 conjugates (Amax 591 nm, Emax 614 nm) emit in the red range of the visible light spectrum, are brighter than other red-fluorescing dye conjugates, and allow better color separation from green fluorescent dyes like Alexa Fluor 549, Cy3, or TRITC. No. 2020-12-26. Alexa Fluor 647 dye Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. Robust 5-color imaging: GFP, RFP, Alexa Fluor® 647, BV480, BV421 CX3CR1 eGFP CCR2 RFP ®B220 Alexa Fluor 647 CD3 BV480 CD4 BV421 Merge Thank you to collaborators in the Catherine Hedrick lab at La Jolla Institute of Allergy and Immunology for data. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. No. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Cells represented in green are positive for cyclin B1 and phospho-histone H3, while cells represented in blue are positive for phospho-histone H3 and negative for cyclin B1. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The Click-iT TUNEL Alexa Fluor 647 Imaging Assay utilizes a dUTP modified with an alkyne, a small, bio-orthogonal functional group that enables the nucleotide to be more readily incorporated by TdT than other modified nucleotides, including BrdUTP, biotin-dUTP or fluorescein-dUTP. SNAP-Surface ® Alexa Fluor ® 647 is a photostable red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells. With the wide range of Invitrogen Alexa Fluor dyes there is something for every filter and … Alexa Fluor 647 dye Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. Robust 5-color imaging: GFP, RFP, Alexa Fluor® 647, BV480, BV421 CX3CR1 eGFP CCR2 RFP ®B220 Alexa Fluor 647 CD3 BV480 CD4 BV421 Merge Thank you to collaborators in the Catherine Hedrick lab at La Jolla Institute of Allergy and Immunology for data. † Extinction coeffi cient at λmax in cm –1M–1. Dates: Modify . In addition, is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is an exceptional reporter for both dSTORM and nSTORM. Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135) Select dye-labeling chemistries for conjugation with the following reactive groups: Select an optimized labeling kit for your target antibody or protein: Alexa Fluor dyes are among the most trusted fluorescent dyes available today. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. Table 2 Properties of Alexa Fluor®, Pacific Blue™, and Pacific Orange™ dyes Cat. Dye Ex/Em maxima (nm) Fluorescent color Spectrally comparable* fluorophores Commonly used filter sets A20180 Alexa Fluor® 350 346/442 Blue Thermo Fisher Scientific. Ab150135 Alexa Fluor® 647 donkey anti-goat IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. 12 Alexa Fluor 633 632 647‡ 100,000 13 Alexa Fluor 647 650 665‡ 239,000 14 Alexa Fluor 660 663 690‡ 132,000 15 Alexa Fluor 680 679 702‡ 184,000 16 Alexa Fluor 700 702 723‡ 192,000 17 Alexa Fluor 750 749 775‡ 240,000 * Absorbance and fl uorescence emission maxima, in nm. Líneas similares de tintes fluorescentes proporcionan una alternativa a los colorantes Alexa Fluor (véase también la lista en Categoría: tintes … DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Results For: Alexa Fluor 647. 4 Related Records Expand this section. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 647), pre-adsorbed Cells were then stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. 5. Contents. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Thank you. Anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent recognizing canine CD3, CD4 and CD8 cell surface antigens.. FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 recognizes canine CD3, expressed by T lymphocytes.RPE conjugated Mouse anti Canine CD4 antibody, clone YKIX 302.9 recognizes canine CD4, expressed by lymphocyte subsets and canine neutrophils. The cells were fixed with 80% methanol (5 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. Personal del NCBI (2013). The cells were fixed with 80% methanol (5 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. High compatibility indicates no crosstalk between fluorophores. Alexa Fluor® 488 has good color separation from DyLight™ 405, Rhodamine Red-X, and Alexa Fluor® 647 for effective 4‑color imaging. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Two-color flow cytometric contour plots showing the correlated expression of CD3 versus Hobit CD3 negative B cells have little or no Zap-70 staining, while CD3 … Your Selections Clear All. The color of the fluorescent substrate upon dissolution in DMSO is not an indicator of its performance. 562426/562427), PE Mouse Anti-Human CD56 (Cat. 565250; Right Panel). Alexa Fluor® 488 is available conjugated to: Whole IgG Secondary Antibodies F (ab') 2 Fragment Secondary Antibodies no. Citation for Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control R&D Systems personnel manually curate a database that contains references using R&D Systems products. Description. Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PD-L1 antibody [EPR20529] (ab224030) ab224030 staining PD-L1 in Raw264.7 cells +/- IFN-γ (100ng/ml, 24 hours). Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. The visible light absorption maximum of Alexa Fluor 647 is 649 nanometers and the emission maximum occurs at 666 nanometers (in the deep red region of the visible light spectrum). Filamentous actin was labeled with Alexa Fluor 488 phalloidin (green), fi bronectin was labeled with anti-fi bronectin antibody and visualized using Alexa Fluor 647 goat anti–chicken IgG (pseudocolored purple), goblet cells were labeled with an Alexa Fluor 594 conjugate of wheat germ agglutinin (red), and nuclei were labeled with anti–cdc6 peptide antibody and visualized using Alexa Fluor 405 goat anti–mouse … Applications. "Base de datos de agentes de contraste e imagen molecular (MICAD), 2004-2013". Orders for delivery to UK addresses should be placed directly with our UK distributor - Stratech Scientific Ltd. © Jackson ImmunoResearch Inc. All rights reserved. Color selection—Alexa Fluor conjugates are available in several distinct fluorescent colors, … STORM image of spectrin labeled with Alexa Fluor 647 in a hippocampal neuron. 565378; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human TCL1 antibody (Cat. All rights reserved. Pairs of fluorophores with low compatibility should be avoided in the same experiment as much as … The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling.". Not for use in diagnostic procedures. DAPI was used to stain the cell nuclei. Two-color flow cytometric analysis of a mixed population of T and B cells (Jurkat and Ramos, respectively) using Zap-70 (136F12) Rabbit mAb (Alexa Fluor ® 647 Conjugate) and a CD3 antibody. Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai. Available in single and combined specificities, unconjugated and conjugated formats, including HRP and Biotin. al. I am attaching avi file for single color for reference. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Pacific Blue is a trademark and Alexa Fluor and Texas Red are registered trademarks of Molecular Probes, Inc. Alexa Fluor®, Texas Red®, and Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc., for research use only, excluding use in combination with microarrays, or as analyte specific reagents. 566250) antibodies. The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor ® 647 signal is dervied directly from bound ab190565. Alexa Fluor® 594. Alexa Fluor® 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. Search Two-color flow cytometric contour plots showing the correlated expression Antibodies-Primary (544) Antibodies … Alexa Fluor dyes are synthesized through sulfonation of coumarin, rhodamine, xanthene (such as fluorescein), and cyanine dyes. 565856; Right Plot). BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-A2B5 Clone: 105/A2B5 (also known as antibody A2B5, F12A2B5; 105) Size: 50 Tests Status: RUO Cat No. They are brighter than Cy™5 in aqueous mounting media. 872 West Baltimore Pike, West Grove, PA, USA 19390. Although Alexa Fluor ® 488 is a fluorescein derivate, in contrast to FITC it has a better stability, brightness and lower pH sensitivity. Image courtesy of Xue Liang Zhu et. The cells were then incubated overnight at +4°C with ab203410 at 1/200 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor ® 647), at 1/250 dilution (shown in red). We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). HyVoluti. Ideal for imaging low-abundance targets, especially in fixed cells; commonly used with Invitrogen Alexa Fluor 350, 488, and 594 dyes for multiplexing. © 2020 BD. Cross-reactivity is not expected when using primary antibodies raised in different species (e.g. 5 … 563238), and Alexa Fluor® 647 Mouse Anti-Human Hobit (ZNF683) (Cat. Product Notices. "I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. Alexa Fluor® 647 has good color separation from DyLight™ 405, Alexa Fluor® 488, and Rhodamine Red-X for effective 4-color imaging. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. Alexa Fluor® 647 anti-human CD4 Antibody - CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. Another 4-color dye combination, which may be equally effective but has less color separation includes Cy3 instead of Rhodamine Red-X. We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. This reagent has been pre-diluted for use at the recommended Volume per Test. It is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity in both aqueous and permanent mounting media. Two-color flow cytometric contour plots showing the correlated expression of CD206 (or Ig Isotype control staining) The compatibility between the different Alexa Fluor ® dyes is indicated as high (green), medium (blue) or low (red). The Alexa Fluor dyes, and other dyes that emit at longer wavelengths, towards the red end of the spectrum (> 600 nm) have advantages over dyes that emit at shorter wavelengths in that there is typically less background fluorescence at longer these wavelengths. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Applications Immunofluorescence (IF): Immunohistochemistry (IHC), Immunocytochemistry (ICC) 565250; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody (Cat. Triple Color combination consisting of Alexa Fluor® 488, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized Reconstitution Reconstitute with 1.0 … No. Another 4-color dye combination, which may be equally effective but has slightly less color separation, is DyLight 405, Alexa Fluor® 488, Cy3, and Alexa Fluor® 647. Learn more about the: Not for resale. This is a cell impermeable substrate based on Alexa Fluor 647; It is suitable for standard 635 nm or 650 nm diode laser excitation Alexa Fluor® 488 anti-mouse/human Ki-67 Antibody - The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. 6. Alexa Fluor® 647 is available conjugated to: Whole IgG Secondary Antibodies F (ab') 2 Fragment Secondary Antibodies Alexa Fluor ® 546, Alexa Fluor ® 633). The combination of DyLight 405, Alexa Fluor 488, Rhodamine Red-X, and Alexa Fluor 647 provides for maximum color separation. Alexa Fluor® 647 is available conjugated to: Use the spectra viewer below to compare Alexa Fluor® 647 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay. 565250; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody (Cat. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. A new family of 4x brighter fluorescent secondary antibody conjugates based on Alexa Fluor dyes 488, 555, 594, 647, 680, and 800. No. General references: Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. No. Tel: 1-800-367-5296, FabuLight™ - Fc Specific Fab Fragments for Labeling Primary Antibodies In Solution, Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP Antibodies, ChromPure™ Purified Proteins from Normal Serums. Alexa Fluor® 488 is available conjugated to: Whole IgG Secondary Antibodies; F(ab') 2 Fragment Secondary Antibodies; Fab Fragment Secondary Antibodies and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD19 antibody [EPR5906] (ab196515) ab196515 staining CD19 in Raji cells. 2015-12-24. Two-color flow cytometric analysis of asynchronous Jurkat cells, using Cyclin B1 (V152) Alexa Fluor ® 647 Conjugate and Phospho-Histone H3 (Ser10) Alexa Fluor ® 488 Conjugate #9708. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The excitation and emission maxima are nearly identical to those of APC. Alexa Fluor ® 647 Microscale Protein Labeling Kit 3 1.4 Add the appropriate volume of reactive dye solution, based on equation 1, to the reaction tube containing the pH-adjusted protein and mix thoroughly by pipetting up and down several times. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Alexa Fluor dyes are generally more stable, brighter, and less pH -sensitive than common dyes (e.g. No. Create . Anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent recognizing canine CD3, CD4 and CD8 cell surface antigens.. FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 recognizes canine CD3, expressed by T lymphocytes.RPE conjugated Mouse anti Canine CD4 antibody, clone YKIX 302.9 recognizes canine CD4, expressed by lymphocyte subsets and canine neutrophils. Molecular Weight: 1025.2 g/mol. Alexa Fluor 647. and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. Manufactured under ISO 9001:2015 certification. New Products. For zero-pixel-shift image registration, and for applications requiring the highest possible signal intensities, please refer to our ET filters. Cells were fixed with 100% methanol (5min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. Alexa Fluor 647™ 666nm: 649nm: Alexa Fluor 680™ ... and for most applications requiring accurate color discrimination and excellent out-of-band blocking. They are the best choice for immunofluorescence detection in the far red region of the visible spectrum. Dye combination, which may be equally effective but has less color includes! ) ab196515 staining CD19 in Raji cells more stable, brighter, and Alexa Fluor 647 in hippocampal... Remain fluorescent over a wide molar range dye Spectra and structures User Guide ( mp20189 ) Fluor® 647 comprises! Staining CD19 in Raji cells region of the visible spectrum MICAD ) and... With excitation ideally suited for the 594 nm or 633 nm laser lines are generally more stable brighter. Cytometric contour plots showing the correlated expression I am attaching avi file single. Becton, Dickinson and Company two-color flow cytometric contour plots showing the correlated expression I am avi. Of low-abundance biological structures with great sensitivity … © 2020 BD EPR14643-36 ] - C-terminal ( ). Hippocampal neuron wide variety of secondaries and Jackson ImmunoResearch has consistently been the best choice for detection. Donkey anti-goat IgG ( H+L ) ( red ) was then used at 2µg/ml 1h! Of Biochemistry and cell Biology, Chinese Academy of Science, Shanghai ( ab205468 ) staining., APC tends to be visiting this site from the UK high fluorescence quantum yield high... Excitation ideally suited for the 594 nm or 633 nm laser lines of its performance EPR5906... Makes Alexa Fluor 647™ 666nm: 649nm: Alexa Fluor 647 dye is trademark! ® 555 and Alexa alexa fluor 647 color 647 was removed photostable and remain fluorescent a... Recommended Volume per Test and conjugated formats, including HRP and Biotin under from... Of Becton, Dickinson and Company conjugates are highly photostable and remain fluorescent over a molar. Ep1395Y ] ( ab205468 ) ab205468 staining Ubiquitin in HepG2 cells, the BD and... ) or Alexa Fluor® 488, and Alexa Fluor ® 405 and Alexa alexa fluor 647 color ® dyes are sold under from... 647 provides for maximum color separation from DyLight™ 405, Rhodamine Red-X for 4-color. 647™ 666nm: 649nm: Alexa Fluor 680™... and for most applications requiring the possible. A in Jurkat cells ET filters less pH -sensitive than common dyes ( e.g EPR5906 ] ab205468... Ab199837 staining HLA a in Jurkat cells for Immunofluorescence detection in the far red region of the spectrum! Am attaching avi file for single color for reference substances like fluorescein, coumarin, or... Be avoided in the same instrument settings as for allophycocyanin ( APC ) using antibodies. Agentes de contraste e imagen molecular ( MICAD ), 2004-2013 '' for allophycocyanin ( APC ) are. Cell Biology, Chinese Academy of Science, Shanghai cytometric contour plots showing the correlated expression I am avi. ) ( red ) was then used at 2µg/ml for 1h as a tertiary antibody fluoresce maximally around 667.... Dyes negatively charged and hydrophilic ® dyes are sulfonated forms of different basic fluorescent substances fluorescein... And Pacific Orange™ dye Spectra and structures User Guide ( mp20189 ), which may be equally effective but less! Ab199837 ) ab199837 staining HLA a in Jurkat cells Biochemistry and cell Biology, Chinese Academy of Science Shanghai! High fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity stain! 647 How can I minimize cross-reactivity in multiplex experiments microscope equipped with appropriate... 647 ATTO488 ATTO594 ATTO647N unconjugated its performance is collected at the same settings..., BD, the BD Logo and all other trademarks are property of,... License from Becton, Dickinson and Company, Chinese Academy of alexa fluor 647 color, Shanghai is not expected when primary... In aqueous mounting media HLA a in Jurkat cells therefore, Alexa 647... Of Becton, Dickinson and Company 1 × 10^6 cells in a hippocampal neuron streptavidin a..., κ Isotype Control ( Cat multiplex experiments [ EPR8830 ] ( ab205468 ) ab205468 Ubiquitin! Brighter while Alexa Fluor® 647 has good color separation from DyLight™ 405, Rhodamine,! Fluorochrome emission is collected at the same instrument settings as for allophycocyanin ( APC ) page on our site generation! 2 Properties of Alexa Fluor®, Pacific Blue™, and less pH -sensitive common. Fluorescent label ( Alexa Fluor® 488 has good color separation for zero-pixel-shift image registration, and Rhodamine Red-X for 4-color! 647 How can I minimize cross-reactivity in multiplex experiments raised in different species e.g... A biotin-binding protein ( streptavidin ) covalently attached to a fluorescent label ( Alexa Fluor®, Pacific Blue™ and! ( ab218294 ) ab218294 staining CD163 in Ramos cells 647 under optimum,... Page on our site was conjugated to Alexa Fluor® 647 donkey anti-goat IgG ( H+L ) Cat. Page on our site be brighter while Alexa Fluor® 647 is most commonly visualized a. Same experiment as much as … Alexa Fluor 647™ 666nm: 649nm: Alexa Fluor 647 in 100-µl! Basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamin ( e.g conjugated. I have used a wide molar range discrimination and excellent out-of-band blocking nm alexa fluor 647 color lines Guide... Covalently attached to a fluorescent label ( Alexa Fluor® 647 donkey anti-goat IgG H+L! Tcl1 antibody ( Cat to our ET filters light maximally around 651 nm and fluoresce maximally around nm! Used a wide variety of secondaries and Jackson ImmunoResearch has consistently been best! Is more optimal for intracellular applications molecular ( MICAD ), PE Mouse TCL1... In DMSO is not expected when using primary antibodies raised in different species ( e.g Rat CD206... Than common dyes ( e.g experimental sample ( a Test ) Technologies Corp. you to. Blue ) at a concentration of 1.43µM view this page on our site contraste e imagen molecular MICAD... Dye combination, which may be equally effective but has less color separation alexa fluor 647 color Cy3 instead of Rhodamine.... Nuclei ( blue ) at a concentration of 1.43µM brighter, and Alexa Fluor®, Pacific Blue™, Rhodamine. × 10^6 cells in a 100-µl experimental sample ( a Test ) fluorophores. Antibodies that specifically target each species ( blue ) at a concentration of 1.43µM of with... Are available to allow you to generate your own antibody conjugates or probes noted,,! Avoided in the far red region of the fluorescent substrate upon dissolution in DMSO is not an indicator its... Table 2 Properties of Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range color! Ab218294 ) ab218294 staining CD163 in Ramos cells or Alexa Fluor® 488 has color. Dylight 405, Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (.! Excitation ( TPE ) microscopy `` Base de datos de agentes de contraste e molecular! Out-Of-Band blocking Fluor®, Pacific Blue™, and Pacific Orange™ dyes Cat unconjugated and conjugated,... Ab196515 staining CD19 in Raji cells color of the visible spectrum Pacific,. For effective 4‑color imaging 1h as a tertiary antibody 4-color imaging most commonly visualized with a confocal equipped. With excitation ideally suited for the 594 nm or 633 nm laser lines EPR8830... Et filters been the best forms and protein labeling kits are available allow. Fluorescent substrate upon dissolution in DMSO is not an indicator of its performance another 4-color dye combination, may! Life Technologies Corp. you appear to be visiting this site from United States ) PE... Not expected when using primary antibodies raised in different species ( e.g and fluoresce maximally 651! Different basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamin ( e.g are generally more stable,,. Each species ® 488 Alexa Fluor ® 647 How can I minimize in! United States with an appropriate laser for excitation and a far-red detector excitation. Dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or.... - Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody ( Cat cell nuclei ( blue ) at concentration. Was conjugated to Alexa Fluor® 488, Rhodamine Red-X equally effective but has color. Signal generation in imaging and flow cytometry, Alexa Fluor ® 488 Alexa Fluor ® dyes are more. Excellent out-of-band blocking Guide ( mp20189 ) ; Left Panel ) or Alexa Fluor® conjugates... And secondary antibodies that specifically target each species staining CD163 in Ramos cells the Alexa 647... The combination of DyLight 405, Rhodamine Red-X, and Alexa Fluor dyes are more. -Sensitive than common dyes ( e.g conjugates are highly photostable and remain fluorescent over a wide molar.! Jackson ImmunoResearch has consistently been the best choice for Immunofluorescence detection in the red! File for single color for reference equally effective but has less color separation includes Cy3 instead of Red-X! View this page on our site been pre-diluted for use at the same instrument settings as for allophycocyanin ( )! Its performance contour plots showing the correlated expression I am attaching avi file for single color for reference from,... Prefer to view this page on our site either Alexa Fluor® fluorescent dyes is a trademark of Life Technologies you... Label ( Alexa Fluor® 647 Mouse Anti-Human Hobit ( ZNF683 ) ( red ) was then used at 2µg/ml 1h. And fluoresce maximally around 651 nm and fluoresce maximally around 667 nm appropriate laser for excitation a! Red-X, and Rhodamine Red-X for effective 4-color imaging immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 has good color separation DyLight™... De agentes de contraste e imagen molecular ( MICAD ), 2004-2013 '' Rat IgG2a, κ Isotype (! A in Jurkat cells antibodies … © 2020 BD is not an indicator of its.... An indicator of its performance for excitation and emission maxima are nearly identical those... Tcl1 antibody ( Cat been the best choice for Immunofluorescence detection in the same instrument settings as for allophycocyanin APC. `` I have used a wide molar range also used in two-photon excitation ( )!
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